The Supporting Information is available free of charge at. Specific protein–protein interactions in the cytoplasm in comparison to SGs determine the partitioning of folding states between the respective phases during HS. In vitro and in silico experiments however suggest that the increased flexibility of the unfolded state constitutes only a minor driving force to associate with the dynamic biomolecular network of the condensate. All amplicons were cloned into the pGEM-T vector (Promega, U.S.A.) and sequenced (three clones for each amplicon) by Microsynth Seqlab (Microsynth. Between the SVZ and IZ, the MAZ is a point of accumulation of polarizing cells. Beyond the VZ, the SVZ contains proliferating and early differentiating neural progenitors. Nevertheless, some factors can affect the successful generation of a high-quality sequence. Located on the basal surface of the cortex proximal to the cerebral ventricles is the VZ responsible for generation of NSCs. Optimized processing steps, proven pro - tocols, and over 25 years of experience in the area of Sanger sequencing usually result in long read lengths and clean sequences. Microsynth (founded in 1989) is a leading company in Europe in the field of DNA/RNA syntheses & analyses. We find that irrespective of aggregation the folding stability determines the association of SOD1 with SGs in cells. Microsynth always strives to get the most out of your DNA sequencing samples. Here, we investigate the association of superoxide dismutase 1 (SOD1) proteins with different folding stabilities and aggregation propensities with condensates in cells, in vitro and by simulation. The folding states that are primarily associated with SGs, as well as the function of the phase separated environment in adjusting the energy landscapes, remain unknown. Stress granules (SGs) are among the most studied membraneless organelles that form upon heat stress (HS) to sequester unfolded, misfolded, or aggregated protein, supporting protein quality control (PQC) clearance.
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